Fluorescent In Situ Hybridization Probe Design

Using spectrally distinct fluorophore labels for each hybridization probe this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display.

Fluorescent in situ hybridization probe design.

The dna probe typically comes from cloned sources such as plasmids cosmids pacs yacs or bacs. The dna probe and the target dna to which the probe will be hybridized. Multiplex fluorescence in situ hybridization fish enables you to assay multiple targets and visualize colocalized signals in a single specimen. New trends in fluorescence in situ hybridization for identification and functional analyses of microbes.

Where the insert may contain a specific gene or originate from a specific chromosomal. Fluorescence in situ hybridization fish involves the preparation of two main components. Curr opin biotechnol. Due to the high allele diversity of genes a single probe is not sufficient to detect all alleles of a gene.

However the well established concepts for oligonucleotide probe design cannot be transferred to polynucleotides. Wagner m haider s. The fluorescent probes are nucleic acid labeled with fluorescent groups and can bind to specific dna rna sequences.